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A recombinant nucleocapsid-based indirect ELISA for Rift Valley fever IgG detection in Humans
The recombinant nucleocapsid-based indirect ELISA is a newly developed assay for the detection of Rift Valley fever IgG
antibodies in humans. Compared to the traditional sandwich ELISA, this new assay offers the following advantages:
- It allows for testing 40 serum specimens in duplicate per ELISA plate compared to 20 specimens in duplicate with the sandwich ELISA
- The indirect ELISA is one step (60 min) shorter than the sandwich ELISA
- The test has high sensitivity (99.72%) and specificity (99.62%)
Standard operating procedure (SOP) Manual
Flow Chart
An inhibition (competitive) ELISA for detection of antibodies to Rift Valley Fever in all Species
Standard operating procedure (SOP) Manual
Flow Chart
Test Protocol
Rift Valley Fever Antigen Detection by ELISA Immunocapture for all Species
Flow Chart
Rift Valley Fever IgG Detection by ELISA in Bovines
Flow Chart
Test Protocol
Rift Valley Fever IgG Detection by ELISA in Livestock
Flow Chart
Test Protocol
Rift Valley Fever IgM Detection by ELISA in Bovines, Sheep and Goats
Flow Chart
Test Protocol
Journal of Virological Methods 157 (2009) Pages 15-24
Journal of Virological Methods 146 (2007) Pages 119-124
Journal of Virological Methods 140 (2007) Pages 106-114
Journal of Virological Methods 127 (2005) Pages 10-18
Journal of Virological Methods 124 (2005) Pages 173-181
Journal of Virological Methods 113 (2003) Pages 103 -112
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